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KMID : 0545120070170111890
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Journal of Microbiology and Biotechnology
2007 Volume.17 No. 11 p.1890 ~ p.1893
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Cloning and Expression of a Parathion Hydrolase Gene from a Soil Bacterium, Burkholderia sp. JBA3
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Kim Tae-Sung
Choi Min-Kyeong
Weon Hang-Yeon
Kim Mi-Sun
Seong Chi-Nam
Song Hong-Gyu
Ka Jong-Ok
Ahn Chae-Hyung
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Abstract
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A bacterium, Burkholderia sp. JBA3, which can mineralize the pesticide parathion, was isolated from an agricultural soil. The strain JBA3 hydrolyzed parathion to pnitrophenol, which was further utilized as the carbon and energy sources. The parathion hydrolase was encoded by a gene on a plasmid that strain JBA3 harbored, and it was cloned into pUC19 as a 3.7-kbp Sau3AI fragment. The ORF2 (ophB) in the cloned fragment encoded the parathion hydrolase composed of 526 amino acids, which was expressed in E. coli DH10B. The ophB gene showed no significant sequence similarity to most of other reported parathion hydrolase genes
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KEYWORD
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Parathion hydrolase gene, Burkholderia sp. JBA3, biodegradation
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